http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2675823-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5ecc01dbbe855d729f0c7d68a3e5cd8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37f4922dfb7777b019e504b885211b8e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-026 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-02 |
| filingDate | 2012-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64375c00aabc98e39a6f393848c97f4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17e9e85195ec481045ed8fbcc05b4049 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc4889464757afc4b4a74b55f4467a2e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_797b95f90068f64c9652a8782d38c6a1 |
| publicationDate | 2013-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2675823-A1 |
| titleOfInvention | Method for preparing peptides by assembling multiple peptide fragments |
| abstract | The invention relates to a method for preparing a peptide assembly of n fragments and of n-1 amino acids having a thiol function, which is represented by formula (I): A1-C1-A2-A3-... -Ci-1-Ai-...-Cn-1-An, where A1, A2, A3... Ai.., An are peptide fragments, C1, C2, C3... Ci-1 Cn-1 are amino acid residues having a thiol function, n is between 3 and 50, and i is 2 to n, said method comprising preparing a peptide thioester of the formula A1SEa (II), where A1 is a peptide fragment and SR is the residue of an alkylated thioester, R being optionally substituted alkyl from a bis(2-sulfanylethyl)amino peptide: A1-SEAoff, where SEAoff is a cyclic bis(2-sulfanylethyl)amino group, by means of a thiol R-SH, in the presence of a cyclic disulfide reducer, followed by condensing on a peptide fragment having the structure: H-C1(SR')-A2-SEAoff (III), where (SR') is a disulfide residue, on the thiol of the C1 amino acid, in the presence of an aromatic thiol ArSH, then converting the resultant novel peptide, having the structure A1-C1-A2-SEAoff (IV), into a peptide-thioester of formula A1-C1-A2-SR (III') by means of a thiol R-SH, in the presence of a cyclic disulfide reducing agent, and condensing on a peptide fragment having the structure H-C2(SR')-A3-SEAoff (III') in the presence of an aromatic thiol ArSH, which yields a peptide fragment having the structure A1-C1-A2-C2-A3-SEAoff (IV), and repeating both of said steps up to n-2 times, so as to obtain a peptide fragment having the structure A1-C1-A2-C2-A3-... -Ci-1-Ai-....-Cn-2-An-1-SEAoff (IVm), then using a native ligation reaction of said resulting peptide fragment (IVm) with a peptide of formula H-Cn-1-An (Vn) by reducing in the presence of a cyclic disulfide reducing agent, which yields the multiple peptide assembly of general formula (I). |
| priorityDate | 2011-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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