| abstract |
A chromatography system (1 ) for separation of a biopolymer is described, comprising at least one feed tank (3), at least one hold tank (4; 4a, 4b, 4c), at least one elution buffer tank (5), at least one eluate tank (6), at least two packed bed chromatography columns (7,8) and for each packed bed chromatography column (7,8) at least one pump 810) and at least one outlet detector (11) both connected to said each packed bed chromatography column (7,8), wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each connected to the packed bed chromatography columns via a system of valves (12), and wherein said hold tank(s) is/are connected to at least one inlet end (13) of a column (7,8) and at least one outlet end (14) of a column (7,8). |