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filingDate 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2013-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-2666857-A1
titleOfInvention Nucleic acid construct for expressing oxidative stress indicator and use thereof
abstract The present invention provides a nucleic acid construct for expressing an oxidative stress indicator comprising: a nucleic acid sequence encoding an Nrf2 protein-derived partial protein that comprises at least an Neh2 domain sequence and substantially lacks or is functionally deficient in an Neh1 domain sequence or an Neh1-Neh3 domain sequence; a stress-inducible promoter sequence positioned upstream of the nucleic acid sequence encoding an Nrf2 protein-derived partial protein; and a nucleic acid sequence encoding a protein capable of generating a detectable signal, the nucleic acid sequence being positioned downstream of the nucleic acid sequence encoding an Nrf2 protein-derived partial protein. The present invention also provides a method for measuring oxidative stress and a method for screening for an anti-oxidative stress agent, using the nucleic acid construct.
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