Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bb28dd172d2102eb407e005f1af8f891 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
filingDate |
2010-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b03502d676c57fe571ab731a0d5ae51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b913e276f6d277e02a2b58d7bd08fe0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43f51ebf01d83855d544a871b7d94ea8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ac9353316f4d0367e824b7bf9837fe5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b57bf798ac32b6dde5db4c74846fd307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9abbf803ba71bbe2874bff1a14caade3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99fd61b663481562f3679e1efd6260ab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90b31227065925f3e17ff00406dd7cba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54ae3936d0d481b7605eccf97b5460ab |
publicationDate |
2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-2598876-A1 |
titleOfInvention |
Novel ultrasensitive cell based sensors and uses thereof |
abstract |
The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a protease as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis, such said granule stored protease reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific cleavage sequence; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection. |
priorityDate |
2010-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |