| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41686a979f961bce6a9855b4b409a5b8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-44 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2011-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c93f8b68cdc954018f4f82e3b296df20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adb0620110c3ab7d5d7eb684b8c67f66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63e2d65990fe0051c1df4f3d83d1b210
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92aa2c0e1f79461fee1d5a5dc2d828cc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfdc523313fe6eb414c0664f5ac00ac6 |
| publicationDate |
2013-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-2547767-A2 |
| titleOfInvention |
Use of achromopeptidase for lysis at room temperature |
| abstract |
A process for detecting the presence or absence of gram-positive bacteria in a biological sample. The biological sample can be obtained from any mammal and contains, at a minimum, cellular components. The sample is combined with an enzymatic lysing agent such as achromopeptidase, and lysed at room temperature. Ferric oxide is then added to the sample containing achromopeptidase. A magnetic field is applied to the sample and nucleic acids are extracted from the cellular components. Target nucleic acids, if present, are amplified using techniques such as Polymerase Chain Reaction (PCR) and then used to detect the presence or absence of gram-positive bacteria. Staphylococcus aureus and Streptococcus agalactiae are examples of target bacteria detected by the methods of the present invention. |
| priorityDate |
2010-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |