| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0ddb9f3656270bc5410be3679322fead |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y106-03 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y106-03001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y111-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0065
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
2011-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_826cd193693ed42ea75ee33216b2b00d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8922aae0d8069af559022f2b61051e14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82556ffc5108df80daee1ccbb96cec87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78e1183228d6fa12b39de28f46f4b015
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33b03db79c6234ee73da8d5bf7ae4202
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a81171496b3a055f4e1d64eec25d820 |
| publicationDate |
2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-2527436-A9 |
| titleOfInvention |
Nadh oxidase mutant having improved stability and use thereof |
| abstract |
Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation. |
| priorityDate |
2010-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |