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filingDate 2010-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d8a8491cab93eee37dacd7a9695814a
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publicationDate 2012-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-2478112-A1
titleOfInvention Method for identifying bacteria from the bacillus cereus group
abstract The present invention relates to the field of microbiological monitoring of food. Said invention relates to a method for identifying bacteria from the Bacillus cereus group and includes the steps that involve: - placing a sample capable of including bacteria of the Bacillus cereus group, a reaction medium including at least one fluorescent phosphatidylcholine-specific phospholipase C substrate, and a Gram-negative bacteria inhibitor in contact with each other in a container; - incubating the above-mentioned sample, reaction medium, and Gram-negative bacteria inhibitor together; and - identifying the bacteria of the Bacillus cereus group by detecting the hydrolysis reaction of the PC-PLC substrate, wherein the pH of the reaction medium and the time required for detecting the PC-PLC substrate hydrolysis reaction used are matched so that said Bacillus cereus bacteriagroup hydrolysis reaction is detected before the hydrolysis of the PC-PLC substrate by the Gram-positive bacteria other than those belonging to the Bacillus cereus group and potentially present in the sample are not detectable.
priorityDate 2009-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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