http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2446017-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2efbe95965e682e179dfd266c9980505 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 |
| filingDate | 2010-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9923c1782be43a9a7141500361b1e36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c67f0e0b30d1b4fd93bb65ea17a4efd4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_421c9749209c59e20fed137c0d3c5702 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2bcca0ab5da88fcffe13ef8159f3172 |
| publicationDate | 2012-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2446017-A2 |
| titleOfInvention | Improvement of ethanol yield and reduction of biomass accumulation in the recombinant strain of saccharomyces cerevisiae overexpressing atp degrading the enzymes |
| abstract | A new approach for increase of ethanol yield during alcoholic fermentation via decrease of biomass accumulation by using ATP degrading enzymes is described. The part of the Saccharomyces cerevisiae SSBl gene coding for cytosolic ATPase domain of ribosome associated chaperon cloned into expression cassette under control of the glycerol-3 -phosphate dehydrogenase gene (GPDl) promoter was introduced into the S. cerevisiae BY4742 strain. The recombinant strains were tested for their ability to grow and produce ethanol during glucose anaerobic and aerobic cultivations. Strains overexpressing ATPase domain of SSBl possessed decreased concentration of intracellular ATP. They accumulated elevated amounts of ethanol and were characterized by decreased biomass accumulation as compared to the wild-type strain under both anaerobic and aerobic conditions. Similarly, the apyrase gene apy from E. coH encoding ATP/ADP hydrolyzing phosphatase and ATPase domain of SSBl gene of S. cerevisiae were co-expressed under the control of galactose-inducible GALl promoter. The recombinant S. cerevisiae strains revealed slight reduction of biomass accumulation, while specific ATPase activity, ethanol accumulation and yield during alcoholic galactose fermentation under semi anaerobic conditions were increased. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109666705-A |
| priorityDate | 2009-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 606.