http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2430158-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T428-2991 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2010-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_261af2d863339762a7527fa10d4a248a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b579032d9c6b0335918b51afff07d4d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f145c714a00419098d2abbe8742b7bf |
| publicationDate | 2012-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2430158-A1 |
| titleOfInvention | Nucleic acid purification method |
| abstract | The present invention relates to a method for purifying a defined amount of nucleic acids from a sample comprising nucleic acid, comprising at least the following steps: a. bringing the sample comprising nucleic acid into contact with a defined amount of a nucleic acid binding phase having the following features (i) the nucleic acid binding phase comprises ligands binding nucleic acids comprising at least one protonizable group; (ii) the nucleic acid binding ligands are present bonded to a carrier; (iii) the nucleic acid binding phase comprises a surface having a low charge density, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid binding phase used; b. binding the nucleic acids to the nucleic acid binding phase at a pH value (binding pH value) below the pK value of at least one of the protonizable groups; c. eluting the nucleic acids at a pH value above the binding pH value, wherein a defined amount of nucleic acids is obtained. The invention further relates to corresponding kits and nucleic acid binding phases that can be used for purifying nucleic acids. |
| priorityDate | 2009-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.