http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2411809-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e5528e04adfb5d2d1256a5b57e0d7c7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate | 2010-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_994fff7b807bb313d3b0e78cc7ee9aa4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a9548b55b49060eb69ae39f7f23b003 |
| publicationDate | 2012-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2411809-A1 |
| titleOfInvention | Luminescence assay method |
| abstract | This invention relates to a bioassay method for detecting and/or quantitating an analyte employing a first group comprising a lanthanide ion carrier chelate and a first recognition element, wherein said lanthanide ion carrier chelate comprises a lanthanide ion carrier ligand and a lanthanide ion; a second group comprising an antenna ligand and a second recognition element; wherein said lanthanide ion carrier chelate binds strongly to said lanthanide, i.e. log K LnL1 is at least 18, where KLΠU refers to the stability constant of the complex between the ion carrier ligand and the lanthanide ion in solution; or said lanthanide ion carrier chelate binds moderately to said lanthanide, i.e. log K LnL2 is at least 12, where K LnL2 refers to the stability constant of the complex between the ion carrier ligand and the lanthanide ion in solution; and an agent complexing said lanthanide ion with an stability constant log K LnL3 , which value is at least 8, between said agent and the lanthanide ion in solution, is additionally employed at a concentration of at least 1 pmol/l. The antenna ligand binds weakly to the lanthanide ion, i.e. the antenna ligand is either monodentate, bidentate, tridentate or tetradentate. Recognition of said analyte by said first recognition element of said first group and by said second recognition element of said second group results in either chelate complementation, i.e. formation of a mixed lanthanide chelate complex through complementation of said lanthanide ion carrier chelate carrying said lanthanide with said antenna ligand, and accordingly in increased fluorescence; or chelate discomplementation, i.e. said lanthanide ion carrier chelate carrying said lanthanide is separated from said antenna ligand, and accordingly in decreased fluorescence. |
| priorityDate | 2009-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 89.