http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2407540-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 |
| filingDate | 2010-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bca928f7ed948447772b28214d45ffdc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3792a16653a6a35b87c25552e0ced15f |
| publicationDate | 2012-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2407540-A1 |
| titleOfInvention | Method for purifying a target nucleic acid |
| abstract | The present invention pertains to method for purifying at least a target nucleic acid from a sample, said method comprising at least the following steps na) incubating the sample with at least one protein-degrading compound, nb) binding the target nucleic acid to a solid phase, nc) eluting the target nucleic acid from the solid phase, nd) incubating the eluted target nucleic acid with at least one protein-degrading compound, ne) binding the target nucleic acid again to a solid phase nf) optionally eluting the bound target nucleic acid from the solid phase. n nIt was surprisingly found that performing a second protein digestion step after the target nucleic acid was bound and eluted from a solid phase before the nucleic acids are rebound to a solid phase is very efficient in reducing remaining protein contaminations in the isolated nucleic acid. |
| priorityDate | 2010-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 159.