| abstract |
The invention relates to a method for preparing adipic acid, comprising - converting alpha-ketoglutaric acid (AKG) into alpha-ketoadipic acid (AKA), - converting alpha-ketoadipic acid into alpha-ketopimelic acid (AKP), - converting alpha-ketopimelic acid into 5-formylpentanoic acid (5-FVA), and - converting 5-formylpentanoic acid into adipic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst.The invention further relates to a heterologous cell, comprising one or more heterologous nucleic acid sequences encoding one or more heterologous enzymes capable of catalysing at least one reaction step in said method. |