http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2379736-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fab5a78161702097f97524ce6e5cdcd7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2009-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aabd89090fba170864a7d34f6d875086 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae066a70e0f77a0777ff3036dafcc2e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4659ce03460f735dfd3f89fe9e54400 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c96f2598d24df4299a924acb52db5de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_504a8125e252342ac39548a80228ec2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0c8805f9fc80d3e598b7bf06673748a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eccbd4135759ec355f1249f5766ece03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac9b8b899a3beafe7fb5415f88a9fad5 |
| publicationDate | 2011-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2379736-A1 |
| titleOfInvention | Method for verifying and/or identifying hormonally effective substances |
| abstract | The invention relates to a method for verifying and/or identifying hormonally effective substances using the pheromone system of yeast, to yeast cells for carrying out the method, and to the use thereof. The method according to the invention comprises the following steps: a) adding yeast culture medium or buffer to a sample which presumably contains a hormonally effective substance, b) thereafter contacting the sample with haploid yeast cells that possess features 1 through 3: 1. the DNA sequence coded for a heterologous hormone receptor is placed under the control of a constitutive promoter, 2. the DNA sequence coded for a yeast pheromone is placed under the control of a promoter that can be regulated by the hormone receptor, 3. a gene that is coded for a receptor for the yeast pheromone is constitutively expressed, wherein the activation of the hormone receptor by the hormonally effective substance leads to the expression and secretion of the yeast pheromone, and c) the physiological and/or morphological changes of haploid yeast cells are detected. |
| priorityDate | 2008-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 585.