| abstract |
The present invention relates to a transgenic fish having at least one genomically integrated expression cassette containing a 5'-regulatory nucleotide sequence responsive to hormones, particularly estrogenic hormones, connected in a functional manner upstream of a nucleotide sequence encoding a reporter protein. The present invention further relates to methods of using the transgenic fish for various purposes, including, for example: n(1) identifying estrogenic endocrine disruptors; n(2) monitoring estrogen-like activity of test samples; n(3) identifying anti-estrogenic endocrine disruptors; and n(4) investigating the effects of endocrine disruptors on liver regeneration. nExpression cassettes, host cells, and transgenic cells of aquatic animals are also disclosed. |