http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2363469-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2534bb9f13460c6429c25acf8df25d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a09ca043d7e596facff1f44b746e2f4d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6491 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
| filingDate | 2009-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf29d4b0abd05b6ec9b05449939dfc78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83d2fdd818342aa5623eca2bbe115c39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85ff02b1172288fa89324704ae844c11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c805ce021ac58ffea534207c02ddfbf9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43d247451535be22028ac95b84c28067 |
| publicationDate | 2011-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2363469-A1 |
| titleOfInvention | Process for producing protein capable of forming inclusion body |
| abstract | A process for preparing an inclusion body-forming protein is provided. A nucleic acid fragment consisting of a nucleotide sequence coding for a modified alkaline phosphatase signal peptide (modified APSP) where leucine at the 13th position in the amino acid sequence shown in SEQ ID NO: 1 is substituted with proline and/or alanine at the 21st position is substituted with the other amino acid, downstream of which nucleotide sequence is bound a nucleotide sequence of a gene of a protein of interest, and a process for preparing an inclusion body-forming protein consisting of the following steps: (1) preparing an expression vector in which a nucleic acid fragment is incorporated consisting of a nucleotide sequence coding for a modified signal peptide downstream of which is bound a nucleotide sequence of a gene of a protein of interest, (2) preparing a host cell transformed with the expression vector that produces an inclusion body-forming protein, and (3) purifying the inclusion body-forming protein from culture obtained by culturing the host cell that produces the inclusion body-forming protein. The above other amino acid is selected from the group consisting of aspartic acid, glutamic acid, lysine, histidine, phenylalanine and tyrosine. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114032252-A |
| priorityDate | 2008-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.