abstract |
Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe-S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe-S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe-S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol. |