| abstract |
The present invention relates to a cofactor regeneration system which is particularly capable of providing sufficient cofactor, such as NADP + , for the enzymatic oxidation of steroids, such as cholic acid (CS), by suitable dehydrogenases, such as hydroxysteroid dehydrogenases (HSDH). to enable on an industrial scale; a method for cofactor regeneration using this system, as well as methods for carrying out enzyme-catalyzed redox reactions with steroid compounds, in particular the enzymatic oxidation of CS, or a method for producing ursodeoxy cholic acid (UDCS) using the cofactor regeneration according to the invention . |