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filingDate 2010-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_269dc53dac821aecd5713ff07987eed0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fec4be6c7170055b29dc7350805c2d68
publicationDate 2011-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-2325330-A1
titleOfInvention METHOD FOR SIMULTANEOUS DETECTION OF VIROIDS PSTVd AND TCDVd
abstract The present invention relates to a method for detecting viroids, comprising: carrying out nucleic acid amplification reaction using RNA from a test plant sample as a template and a primer set comprising (i) a reverse primer of a sequence of 16 to 30 nucleotides in length within the sequence complement of the nucleotide sequence ranging from nucleotide positions 18 to 114 of a DNA sequence corresponding to the PSTVd genome (PSTVd genome-corresponding sequence); (ii) one or more forward primers for detection of PSTVd of 16 to 30 nucleotides in length, which are designed on the genome-corresponding sequence to locate the 3' end within the region ranging from nucleotide positions 127 to 147 in the PSTVd genome-corresponding sequence; and (iii) one or more forward primers for detection of TCDVd of 16 to 30 nucleotides in length, which are designed on the genome-corresponding sequence to locate the 3' end within the region ranging from nucleotide positions 210 to 224 of the PSTVd genome-corresponding sequence; and determining the presence or absence of nucleic acid amplification by the reverse primer and the forward primer for detection of PSTVd and of nucleic acid amplification by the reverse primer and the forward primer for detection of TCDVd to detect viroid PSTVd and TCDVd in the test plant distinguishing between them.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-303801-B6
priorityDate 2009-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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