http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2325330-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_887c2a042155106b3d0e20bb8e1172da |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2010-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_269dc53dac821aecd5713ff07987eed0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fec4be6c7170055b29dc7350805c2d68 |
publicationDate | 2011-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-2325330-A1 |
titleOfInvention | METHOD FOR SIMULTANEOUS DETECTION OF VIROIDS PSTVd AND TCDVd |
abstract | The present invention relates to a method for detecting viroids, comprising: carrying out nucleic acid amplification reaction using RNA from a test plant sample as a template and a primer set comprising (i) a reverse primer of a sequence of 16 to 30 nucleotides in length within the sequence complement of the nucleotide sequence ranging from nucleotide positions 18 to 114 of a DNA sequence corresponding to the PSTVd genome (PSTVd genome-corresponding sequence); (ii) one or more forward primers for detection of PSTVd of 16 to 30 nucleotides in length, which are designed on the genome-corresponding sequence to locate the 3' end within the region ranging from nucleotide positions 127 to 147 in the PSTVd genome-corresponding sequence; and (iii) one or more forward primers for detection of TCDVd of 16 to 30 nucleotides in length, which are designed on the genome-corresponding sequence to locate the 3' end within the region ranging from nucleotide positions 210 to 224 of the PSTVd genome-corresponding sequence; and determining the presence or absence of nucleic acid amplification by the reverse primer and the forward primer for detection of PSTVd and of nucleic acid amplification by the reverse primer and the forward primer for detection of TCDVd to detect viroid PSTVd and TCDVd in the test plant distinguishing between them. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-303801-B6 |
priorityDate | 2009-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 85.