http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2321424-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e3c02833bd037dc982a7e8590198621c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c50ba74a4b932f47bf6d9e624a05456 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-025 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2009-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3851048b6b615b0cfb1a6c3a8ca38dc7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_816235423be9ff3f9960209865077466 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f396603c3a9c6e19d2f3ac1f878bc172 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21a661911c34eb120fef205389330b8e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2960aff353a690126351f434a84e2bed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b99ce6daad07bdaacefb942b2f071bea |
| publicationDate | 2011-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2321424-A1 |
| titleOfInvention | Determining the sensitivity of a cell to a drug |
| abstract | The present invention provides an in vitro method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug, wherein the method comprises the steps of: (i) treating a patient sample or cell line, or a portion thereof, with a deoxyribonucleoside kinase-dependent drug; (ii) lysing the cells of the patient sample or cell line from step (i); (iii) optionally, mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for deoxyribonucleoside kinase; (iv) mixing a portion of the cell lysate from (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase and a deoxyribonucleoside kinase transcription promoter; (v) mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase, a deoxyribonucleoside kinase transcription promoter and a dephosphorylating agent; and (vi) measuring the bioluminescence of each of the mixtures from steps (iii) to (v), wherein the comparative levels of bioluminescence of each of the mixtures provides a measure of the resistance or sensitivity to the drug. |
| priorityDate | 2008-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 292.