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filingDate 2010-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2011-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-2314669-A1
titleOfInvention Carbon matrix substrates for obtaining biofertilising bacteria
abstract A process for obtaining in vitro bacterial biomasses comprising (i) contacting a soil sample with a matrix substrate to form a zone analogous to that associated with the clay-humic complex (CAH) in order to retain the surface or in the matrix substrate the bulk of the endogenous bacterial microflora to the original edaphic media, (ii) the maturation of this zone allowing the constitutive cells of the CAH which colonized this matrix substrate to migrate to the liquid phase the supernatant (iii) recovery and culture of bacterial strains. The semi-solid carbon matrix substrate consists of a non-bactericidal cellulosic matrix impregnated with demineralized and sterile water placed at the bottom of a sterile box, the quantity of water and of matrix being advantageously the same in order to constitute a semi-solid matrix initially containing 50% dry matter (solid) and 50% water (liquid). An aliquot of diluted soil will be added to approach a pHBA / oses ratio of about 3 to 4, while ensuring that the ose content is initially less than 70 -100 μM. This a priori carbonaceous matrix substrate free of free oses and free osids assimilated by the soil bacteria gradually releases during its degradation, the telluric microflora provided via the soil sample ensuring the enzymatic and / or fungal degradation. of the cellulosic matrix. The particle size of the fine soil particles is about 70 to 170 μm. The proportions matrix substrate: soil sample are advantageously about 5.
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