| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bcb146c68b67089013b4c7a614504254 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-90241 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-12007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2000-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e158c829c10dbf676b0feabdb90dc99
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2017d0faf17d2cb78d2dfdf30a61550a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_353dd89c042c111b82720e47c3897036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60d8cf8b963e2a733060df8586fcce41
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d9ea43c2af24e17fa980fe315b4b332 |
| publicationDate |
2011-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-2284271-A2 |
| titleOfInvention |
Mutant luciferase |
| abstract |
A recombinant protein having luciferase activity and at least 60% similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in Photinus pyralis luciferase is mutated as compared to the corresponding wild-type luciferase, such that the luciferase enzyme is able to emit light at a different wavelength as compared to the corresponding wild-type luciferase and/or has enhanced thermostability as compared to the corresponding wild-type luciferase. In general, the residue corresponding to 357 in Photinus pyralis luciferase is changed from an acidic amino acid to a non-acidic amino acid and preferably an uncharged polar amino acid such as tyrosine. n Mutant luciferases in accordance with the invention can produce a large (50nm) wavelength shift in emitted light and have good thermostability. The resultant colour shift can be reversed by addition of coenzyme A. n These properties make the mutant particularly useful in a variety of assays. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9927430-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9840730-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102191213-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102191213-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11661623-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8809529-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9139836-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9790537-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9487520-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10774364-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9938564-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9951373-B2 |
| priorityDate |
1999-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |