| abstract |
A method for efficiently amplifying abnormal prion protein (PrP Sc ) derived from bovine spongiform encephalopathy (BSE) is provided. Ultimately, the invention aims at eradicating the transmission of a prion disease by detecting a BSE-infected cow early and developing a method for inactivating prions and permitting early examination of prion inactivation. Provided is a method for efficiently amplifying PrP Sc derived from BSE, wherein the method is based on a PMCA (protein misfolding cyclic amplification) method in which normal prion protein (PrP C ) is used as a source and PrP Sc is used as a seed, and PrP Sc derived from BSE is amplified by stir-mixing, incubating, and sonicating both the PrP C and the PrP Sc repeatedly, and wherein the method includes performing stir-mixing-incubation-sonication in the presence of a polysaccharide sulfate. |