| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9779afa74a3c3f6ff93275907bfe44f3 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 |
| filingDate |
1999-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28852686c0755ec7ca69dc8e5a28b393
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4817aac44043ba811ede1fdeeb76a4f |
| publicationDate |
2010-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-2267127-A1 |
| titleOfInvention |
Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| abstract |
The present invention provides rapid and highly effective methods for extracting nucleic acids suitable for PCR amplification from tissue samples and paraffin-embedded tissue samples. Extraction is accomplished within a few minutes using a composition comprising a buffer, at least one nonionic surfactant, and a protease enzyme. The sample is then heated at alkaline pH and after a centrifugation step, the DNA is the supernatant can be used directly in known amplification methods, such as PCR. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11299726-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10233440-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10465182-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113265444-A |
| priorityDate |
1998-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |