| abstract |
Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing deubiquination, activity using resonance energy transfer (RET) are provided. nEither a substrate is linked to donor and acceptor (at least one of which is a fluorescent polypeptide) or a substrate is linked to either donor or acceptor and the other FRET partner is present on a binding partner capable of recognising the ubiquitination state of a substrate. Corresponding fusion polypeptide constructs are claimed as products. |