| abstract |
The present invention provides a creatinine amidohydrolase with more improved resistance to chelating agents or more improved specific activity, which overcomes the disadvantages of known creatinine amidohydrolase. The present invention provides a method for improving the chelating agent resistance of creatinine amidohydrolase, the method including substituting an amino acid in at least one position selected from position 175, position 124, position 78, and position 122 in the amino acid sequence of creatinine amidohydrolase derived from Pseudomonas putida, or in at least one position equivalent thereto, with another amino acid residue; and a method for improving the specific activity of creatinine amidohydrolase, the method including substituting an amino acid at position 43 in the above-mentioned amino acid sequence, or at a position equivalent to position 43, with another amino acid. |