http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2175018-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b8c8578d7946278673732b229d6af1f9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2008-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52076188dc07a7bb9f25be7b9993cedc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f15978b9e9aa4ebb31f887862c775e6b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b005883e133a72c1ff67914a2c912370 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_635afdeae5ab8a8fc905ee61fbc70655 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96f09f349659af7cccab78b3b7d597f9 |
| publicationDate | 2010-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2175018-A1 |
| titleOfInvention | Process of clean cloning |
| abstract | A process of inserting a nucleic acid sequence of interest into an acceptor nucleic acid, comprising the following steps: namplifying by PCR a DNA comprising in the following order a sequence segment U, a nucleic acid sequence segment of known nucleotide sequence K2 and a nucleic acid sequence segment of known sequence K3 using a forward primer defining a first end of the amplified DNA and a reverse primer defining a second end of the amplified DNA, said reverse primer terminating at its 3'-end in a nucleotide sequence of nucleic acid sequence segment K3; ntreating the linear double-stranded DNA molecules contained in the PCR product obtained in the previous step with an exonuclease to obtain a single-stranded overhang at the first end of the DNA and a single-stranded overhang comprising nucleic acid segments K2 and K3 at the second end of the DNA; nannealing the product of the previous step to a linearized double-stranded acceptor nucleic acid having at a first end thereof a single-stranded overhang complementary to the single-stranded overhang of the first end of the DNA and at a second end thereof a single-stranded overhang complementary to the single-stranded sequence segment K2 of the second end of the DNA; and ntransforming the reaction product obtained in the previous step into a host cell. |
| priorityDate | 2008-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.