http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2161337-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec5b8c0fc1f827c1360a8f5ea5652225 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2009-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d926d9674e85d3a919857833434f6ceb |
| publicationDate | 2010-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2161337-A1 |
| titleOfInvention | Process for isolating nucleic acids from a nucleic acid-containing sample and a testkit |
| abstract | The present invention relates to a method for isolating nucleic acids from nucleic acid-containing samples, wherein the samples are mechanically digested by known methods and the method is characterized by the following steps: - Addition of a chaotropic denaturant to the sample Homogenize the lysed sample and remove cell debris by centrifugation - Addition of alcohol - Separation of the nucleic acids by binding the nucleic acids to a mineral carrier - To wash - Addition of Na citrate / NaCl solution Washing and elution of the adsorbed total nucleic acid according to known methods.n n n The invention further relates to a test kit for isolating nucleic acids from nucleic acid-containing samples, comprising a) buffer containing guanidine hydrochloride, guanidine isothiocyanate or guanidine thiocyanate, b) ethanol, c) separation column for nucleic acids, d) washing buffer, f) Na citrate / NaCl solution, g) collecting vessels |
| priorityDate | 2008-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.