| abstract |
The present invention relates to improved methods for the subsequent transformation of at least two DNA constructs into the genome of a Zea mays plant by means of Agrobacterium -mediated transformation, comprising the steps of a first transformation with a first construct comprising a mutated ahas selection marker gene, conferring resistance to imazethapyr but sensitive to imazaquin, and selecting plants resistant to imazethapyr, and a second transformation with a second construct comprising a second mutated ahas selection marker gene conferring resistance to both imazethapyr and imazaquin, and selecting plants resistant to imazaquin. |