http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2147107-B1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5098eff6d67391cd857fae0aa3174bcb |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-685 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
filingDate | 2008-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2011-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a4a5e4ad0565a61255cf2bb923b3408 |
publicationDate | 2011-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-2147107-B1 |
titleOfInvention | Expression cloning method suitable for selecting library clones producing a polypeptide of interest |
abstract | The present invention relates to methods for producing a recombinant polypeptide of interest, the method comprising the steps of: a) providing a polynucleotide library encoding one or more polypeptides of interest, wherein the library was prepared in an expression cloning vector comprising at least the following elements: i) a polynucleotide encoding a selectable marker; ii) a fungal replication initiation sequence, preferably an autonomously replicating sequence (ARS); and iii) a polynucleotide comprising in sequential order: a promoter derived from a fungal cell, a cloning-site into which the library is cloned, and a transcription terminator; b) transforming a mutant of a parent filamentous fungal host cell with the library, wherein the frequency of non-homologous recombination in the mutant has been decreased compared to the parent; c) culturing the transformed host cell obtained in (b) under conditions suitable for expression of the polynucleotide library; and d) selecting a transformed host cell which produces the polypeptide of interest. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013163590-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013119302-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020173817-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3219794-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3279320-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018050666-A1 |
priorityDate | 2007-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1368.