http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2145006-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f8661a2b8c333cac1d36a198376e880c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 |
| filingDate | 2008-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ec8244a9b4c3c33b5e20e7f220cf888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21b7c2fb20e6a0fb650f26f513fb2136 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_975e8644690ace604a82421ffff0c567 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0eaa43b9ceea58fd1bc9d6d601cf5949 |
| publicationDate | 2011-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2145006-B1 |
| titleOfInvention | Expression system used for the antibiotic-free production of polypeptides |
| abstract | The invention relates to an expression system for the production of one or several target polypeptide(s), comprising a host cell, in whose genome the DNA sequence coding the glycerine-3-phosphate dehydrogenase is inactivated or partially or fully deleted and which is transformed with an extrachromosomal element that comprises a DNA Sequence coding the target polypeptide(s) and the glycerine-3-phosphate dehydrogenase. According to the invention, both the host cell genome and the extrachromosomal element do not carry any antibiotic-resistant gene. The invention also relates to a DNA sequence coding for a polypeptide with glycerine-3-phosphate dehydrogenase activity, characterized in that the DNA sequence is selected from a) DNA sequences comprising a nucleotide sequence according to SEQ ID NO:1; b) DNA sequences comprising a nucleotide sequence represented by the nucleotides 1338 to 2375 of SEQ ID NO:1; c) DNA sequences coded by the plasmid pTP01 with the plasmid card according to figure 4 and filed under the deposition number DSM 18890; d) DNA sequences coding for the protein sequence according to SEQ ID NO:2; e) DNA sequences which hybridize under stringent conditions with one of the DNA sequences listed under a), b), c) or d); f) DNA sequences which are related to the DNA/nucleotide sequences listed under a), b), c), d) or e) because of the degeneration of the genetic code; and g) complementary strands to the sequences listed under a) to f). The invention further relates to the protein coded thereby. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023083806-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3926039-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021254832-A1 |
| priorityDate | 2007-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.