http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2099820-A2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_114e4423ec77cbc8dab9d0a98909704e |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01004 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B15-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B15-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B30-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 |
filingDate | 2007-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df4344834c37b12317cce9a647e29e36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d07363240a1884a5baedbb7509065d88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35ff43d3b8800cb587a48f3ef74bdc62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fbe11281a7fdf40d97dcfadaef3b594 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_167cd8ef98298d81517380d66eca9fa2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70849a197d596d757fc4a7fcaf79b78a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4b1fa574fdf45e08329b05e5d7e1226 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_741ac6eb472d17a2416ef57280e5a1c6 |
publicationDate | 2009-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-2099820-A2 |
titleOfInvention | A recombinant meso-active thermo-stable protein and the process of design and biosynthesis thereof |
abstract | The present invention deals with examination of the alterability of part, or whole, of the surfaces of beta sheet-based protein structures, focusing especially on enzymes. The alteration is done by supplanting/transplanting a part, or whole, of the surface of one protein onto the surface of a homologous protein of superimposable polypeptide backbone, by exploiting the structural features of beta sheets to alter only the regions of the surface involved in substrate/ligand binding and catalysis. The transplantation involves replacement of a selected set of non-contiguous residues constituting the surface regions desired to be altered in one enzyme/protein, by a set of non-contiguous residues located at analogous positions in the other enzyme/protein, in a manner that is likely to facilitate folding and function of the new protein formed by combining residues from both enzymes/proteins. The present invention also deals with using this surface engineering approach to selectively combine enzyme/protein characteristics from different domains of life that are not ordinarily combined by natural evolution, such as the creation of novel proteins that retains the bulk of the thermostable scaffold of a thermophile enzyme onto which the active surface of a mesophile homolog is transplanted, so as to create a thermo-stable protein with meso-active functional characteristics of pH and temperature of optimal function. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114317838-A |
priorityDate | 2006-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.