Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7254887bc4324c447a6a7cfda1e6b29c |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 |
filingDate |
2007-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2011-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_748660aec59c739297ffee80ef86ad91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b78471105cb13a7f89ee2d34bddafa8f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6ca39235523b49be9a56111b7a788eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16155e98bfea2d6e1897338a80d00af3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e73fcedd88a53193237a482e4b7fe8da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88ed0aa4876e82b65b89c510af6b27d3 |
publicationDate |
2011-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
EP-2087118-B1 |
titleOfInvention |
A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same |
abstract |
Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2548806-C1 |
priorityDate |
2006-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |