http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2075245-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_86289527b50fd2a67582530a0dd8d129 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C225-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B57-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C237-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C237-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C225-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2008-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39969d2680fab187a8543fa7bfae9095 |
| publicationDate | 2009-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2075245-A2 |
| titleOfInvention | Two-photon probe for real-time monitoring of intracellular calcium ions, method for preparing the probe and method for real-time monitoring of intracellular calcium ions using the probe |
| abstract | A two-photon probe for real-time monitoring of intracellular calcium ions is provided. The two-photon probe is very suitable for real-time imaging of intracellular calcium ions, shows 20-50-fold TPEF enhancement in response to Ca 2+ , has a dissociation constant ( Kd TP ) of 0.14 ± 0.02 to 0.25 ± 0.03 µ M, and emits 5-fold stronger TPEF than currently available one-photon fluorescent Ca 2+ probes. Unlike the previously available probes, the two-photon probe can selectively detect dynamic levels of intracellular free Ca 2+ in live cells and living tissues without interference from other metal ions and from the membrane-bound probes. Moreover, the two-photon probe is capable of monitoring the calcium waves at a depth of 100-300 µm in live tissues for 1,100-4,000 s using two-photon microscopy (TPM) with no artifacts of photo-bleaching. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular calcium ions using the two-photon probe. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104371707-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020178177-A1 |
| priorityDate | 2007-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.