http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2069542-A2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dc324aeca4969a84dfe3d9c42130b9be http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cce5542b865eb96284fd89c527401b36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_92208b429d3f81680a850da1e2492997 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0604b66354ef9517619d6ea8a3035d30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b415ded165bcbce8f09ed33d5b8d760f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e91e0184cc865b8e69f4f520b13fea7 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-045 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate | 2007-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da385eadd9e8b22f9599367ca0f37329 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_335f789db7c87df4ffb431fe05a90d70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_232ee8bdfb4309826fe20bc69c42ea84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c47187bb6e639cd5201874992bcc0572 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e78e330f75363b7cee5b254123593289 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57cf737e9fc36679c9f137ae68b12f8c |
publicationDate | 2009-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-2069542-A2 |
titleOfInvention | Methodology for detection, enumeration, propagation and manipulation of bacteriophages |
abstract | A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature- independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages. |
priorityDate | 2006-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.