http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2058392-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5e5c121269689cbab9b3a4548b15caf3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2007-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e712eb496a376b9e32cfc4db42c443af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2beae04573c88529305c9265378c95c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59b0b34aa7d545beeb474b5824481731 |
| publicationDate | 2011-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2058392-B1 |
| titleOfInvention | Dna fragment used in the form attached to 5'-terminus of primer for use in amplification reaction of nucleic acid, and use thereof |
| abstract | The object is to provide a method for confirming the amplification of a nucleic acid rapidly, in a simple manner, at low cost and at high sensitivity after an amplification reaction of the nucleic acid. Specifically, disclosed is a DNA fragment which is a single-stranded DNA fragment having a hairpin structure formed therein, wherein a bulge structure is formed in the hairpin structure. The DNA fragment is intended to be used in the form attached to the 5'-terminus of a nucleic acid amplification primer. Also disclosed is a method for confirming the amplification of a nucleic acid, which comprises conducting a nucleic acid amplification reaction such as PCR by using a hairpin primer having the DNA fragment attached to the 5'-terminus thereof, and measuring the amount of the hairpin primer by using a fluorescent molecule having a bulge structure attached thereto. For example, the method for confirming the amplification of a nucleic acid can be applied to an allele-specific PCR to detect an SNP rapidly, in a simple manner, at low cost and at high sensitivity. |
| priorityDate | 2006-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.