http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2058391-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69b4de7d64ebafc368b849bca94eab18 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2820-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-108 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
filingDate | 2007-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b317da85a90841c4e29f413cd9e5fb28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e1d407dddf3a133fafd4028f09a9049 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24757450ff12c5ea46d4bfe3a15205cc |
publicationDate | 2009-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-2058391-A1 |
titleOfInvention | Method for highly amplifying target gene in mammalian cell and vector therefor |
abstract | A vector of the present invention is a vector for amplifying a target gene in a mammalian cell, the vector including an amplification-activating fragment, which is a partial fragment of a mammalian replication initiation region and has a gene amplification activity site, and a mammalian nuclear matrix attachment region. In the case where the mammalian replication initiation region as described above derives from a c-myc locus, for example, the above-described partial fragment at least contains a duplex unwinding element and a topoisomerase II-binding domain. The vector as described above improves gene transfer efficiency and gene amplification efficiency compared with the existing high gene amplification systems. Thus, a method whereby a "high gene amplification system" developed by the inventors can amplify a target gene with better gene transfer efficiency and a vector to be used in this method are provided. |
priorityDate | 2006-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 337.