http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2027289-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4177fb56fc658037753da3c3625fd5fd |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J19-00 |
| filingDate | 2007-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58d0671430a22bfca14afcc7de72b65a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43a3fa090c0f9b192c5f32234f7e5048 |
| publicationDate | 2009-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2027289-A1 |
| titleOfInvention | Design of capture molecules for the detection of amplicons with high sensitivity |
| abstract | The invention relates to a method for the design and/or the preparation of a polynucleotide capture molecule for detecting an amplicon having one strand serving as target to be detected and/or quantified after hybridization on said capture molecule, comprising the steps of : a) selecting a primer pair defining the amplicon; b) selecting a specific sequence of 10 to 100 nucleotides within the amplicon, such that said specific sequence defines two non-complementary ends of the amplicon; c) defining the capture molecule having a capture portion that is complementary to the specific sequence selected in step b), and a spacer portion comprising at least 20 nucleotides; d) identifying among the two non- complementary ends of the amplicon a spacer end and a non-spacer end, respectively, such that the spacer end is non-complementary to the spacer portion of the capture molecule, and said spacer end exceeds said non-spacer end by at least 50 bases. |
| priorityDate | 2006-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.