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filingDate 2007-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2009-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-2013334-A2
titleOfInvention Method for expansion of human corneal endothelial cells
abstract A method for expanding human corneal endothelial cells includes: (a) providing an amniotic membrane with or without amniotic cells, wherein the amniotic membrane has an extracellular matrix; (b) placing onto the amniotic membrane, a sheet of endothelial layer, or a cell suspension including human corneal endothelial stem cells; and (c) culturing the corneal endothelial cells on the amniotic membrane for a duration sufficient for the corneal endothelial stem cells to expand to an appropriate area. The invention also relates to a method for creating a surgical graft for a recipient site of a patient using the method for expanding human corneal endothelial cells, and the surgical graft prepared therefrom.
priorityDate 2006-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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