http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2010659-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5abb24a5aba0f2be9b494f2062a10678 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01056 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01057 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-0705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07019 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-317 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1241 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-111 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1072 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1075 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2007-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ae5866a94d2882e45e880faa4e43834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adc985cdfe1f9ad5744fb6d5d623c519 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f771addfabd2dc9ca50e7bc9e99dfa10 |
| publicationDate | 2009-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-2010659-A2 |
| titleOfInvention | Kits and methods for generating 5' capped rna |
| abstract | The present invention relates to kits and methods for efficiently generating 5' capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified- nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production o 5'-capped RNA having a modified cap nucleotide and for in vitro or in vivo production o polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5'-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide- capped RNA also provides methods and kits for obtaining only type-specific or condition- specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labelin uncapped RNA comprising primary RNA transcripts or RNA having a 5 '-diphosphate with detectable dye or enzyme moieties. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8846348-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3446712-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9115380-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9005930-B2 |
| priorityDate | 2006-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 487.