| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca32fd2a44fd326ffd027a75a57bd13f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_074e5ad01c7febb4949baddd071d611d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-23
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-35
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1088
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01018
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-56 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate |
2007-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e026ff7992f3de60ea25eda080119a87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab7a16d0d18b2c3530173003661039f7 |
| publicationDate |
2008-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-2000480-A1 |
| titleOfInvention |
Method for the production of high-level soluble human recombinant interferon alpha in e. coli and vectors useful for such a production |
| abstract |
Method for the production of high-level soluble human recombinant interferon alpha protein (rhuIFNα) in E. coli and vectors useful for such a production. n Said method comprises the steps of: n(1) Transforming an E. coli selected in the group consisting of E. coli protease deficient host strains, and E. coli reductase deficient host strains, with a recombinant expression vector comprising the sequence encoding the glutathione-S-transferase (GST), a junction sequence including a recognition site for a specific protease and a sequence able to encode an interferon alpha (IFN alpha) protein under the control of an inducible promoter, said vector encoding a GST-IFN alpha fusion protein n(2) Expressing said interferon alpha protein in conditions comprising the induction of the expression with 0.1 mM-0.5 mM IPTG and a growth temperature of 25° and/or 37°C, depending on said E. coli strain and n(3) Isolating the expressed IFN alpha protein. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2527424-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012160027-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/MD-4384-C1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-027218-B1 |
| priorityDate |
2007-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |