http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1988176-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bfdf49224a405be1f7b653c56ebad94 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11fab36a5b07a7084f7748a44eccd82d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0723e5fdfc19e9442fec95968453c1f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f746bc496acd7458f276a3941935013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e660f5252e84b7b5dba9ac7a198c0295 |
| publicationDate | 2008-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1988176-A1 |
| titleOfInvention | Method of detecting acid-fast bacteria using ribosomal RNA as target |
| abstract | A detection method using an RNA amplification step amplifying the 16S rRNA of Mycobacterium tuberculosis which comprises synthesizing a cDNA by the action of an RNA-dependent DNA polymerase by using a specific sequence in the 16S rRNA of Mycobacterium tuberculosis present in a sample as a template, degrading the RNA strand in the resulting RNA-DNA double strand by an enzyme having a ribonuclease H activity to give a single-stranded DNA, forming a double-stranded DNA having a promoter sequence which can be transcribed into an RNA molecule homologous or complementary to the specific sequence by using the single-stranded DNA as a template by the action of a DNA-dependent DNA polymerase, and then transcribing the double-stranded DNA into an RNA transcript, which can act as a template in the subsequent cDNA synthesis by the RNA-dependent DNA polymerase, wherein a first primer containing a sequence homologous to part of the 16S rRNA of Mycobacterium tuberculosis to be amplified and a second primer containing a sequence complementary to part of 16S rRNA of Mycobacterium tuberculosis to be amplified (either of which additionally has a promoter sequence for the RNA polymerase at the 5' end) are used, wherein said first primer has the sequence of SEQ ID NO:1, and said second primer has the sequence of SEQ ID NO:2. |
| priorityDate | 2002-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 114.