http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1957670-B8
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_22d804b31f31ac059eda1f288f7dda0e |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2006-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2010-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9088458d9e600e24098c8853f97339e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c64a1e067d62c008367777d1e034e10d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd6c5aeeb95da87f670fc5b82272a7fc |
publicationDate | 2010-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1957670-B8 |
titleOfInvention | Homogeneous luminescence bioassay |
abstract | This invention relates to a homogenous bioassay for use in measurement of biological activity, its modulation or analyte concentration of a sample, said bioassay comprising a first group comprising an acceptor, which acceptor is a short lifetime fluorescent compound capable of energy transfer, and a second group comprising a quencher, which quencher is capable of energy transfer from an acceptor. The increase or decrease, respectively, of fluorescence of said acceptor due to the decrease or increase, respectively, of energy transfer from said acceptor to said quencher resulting from lengthening or shortening, respectively, of the distance between said acceptor and quencher is measured. Characteristic for the invention is that the bioassay comprises a further third group comprising a donor for energy transfer to said acceptor, which donor is an up conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound; and said first group comprises a tag, said third group comprises a binder, and said binder has a high affinity for binding to said tag. The fluorescence of said acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor. |
priorityDate | 2005-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.