| abstract |
A method of screening whether or not a test substance can change the cell-stimulating activity mediated by GPR40 characterized by comprising using the test substance, a biomembrane containing GPR40 or a cell containing the same and phospholipase or its salt. According to this method, a substance relating to insulin secretion can be screened. According to this method, moreover, it is possible to screen a substance which is useful in preventing or treating diabetes, diabetic complications and degenerative diseases, hyperglycemia, polyuria, ketonemia, acidosis, insulin resistance, impaired glucose tolerance, neurodegenerative diseases, insulinoma, cancer, hyperinsulinemia, hyperglyceridemia, fatty liver, hypoglycemia caused by insulin oversecretion, arteriosclerosis, hyperlipemia, cerebral stroke, obesity, various diseases induced by diabetes or obesity and so on. |