| abstract |
A method of expressing a hemoglobin, comprising: n(a) providing a host cell containing nucleotide sequences encoding globins capable of associating to form a functional hemoglobin, wherein a nucleotide sequence encodes a β globin having at least one mutation or combination of mutations selected from the group consisting of D73I, D73M, D73E, D73T, D73K, D73L, K82E, K82G, K82P, K82Q, K82S, K82N, K82H, N102A, N102V, K82D (Providence) + D73G (Tilberg), and K82D (Providence) + D73Y (Vancouver); n(b) inducing the host cell to express the hemoglobin; and n(c) purifying the hemoglobin from the host cell. |