| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1cded1dd9dcc9dac6c1d9e44d8c1f78f |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-381
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-96 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-168
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-361
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-363
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-424
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-38
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-26 |
| filingDate |
2006-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84b0bf31dc4fbdeba7027d5312ac26cf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c7159f1c042bbd339143b895c3538da |
| publicationDate |
2008-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1948677-A1 |
| titleOfInvention |
Method for pre-fractionation of complex samples |
| abstract |
The present invention relates to a method for pre-fractionation of complex protein and/or peptide samples. The method comprises the following steps: a) loading the sample onto pre-packed media able to separate proteins/peptides according to their pI-value; b) eluting the media by centrifugal force under denaturing conditions with at least three buffers having different pH in a stepwise manner to obtain at least three fractions separated according to pI-value; and c) subjecting each fraction to further separation in a pH-range corresponding to the pI-values of said fractions. The invention also relates to a kit comprising at least one spin column or multiwell plate filled with chromatography medium able to separate proteins/peptides according to pI-value, at least three buffers, and one or more IPG strips having a narrow pH-range adapted to each of said buffers. |
| priorityDate |
2005-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |