| abstract |
A method for the detection of 5-methylcytosine in genomic DNA samples is described. First, a genomic DNA from a DNA sample is chemically reacted with a reagent, whereby 5-methylcytosine and cytosine react differently, and then the pretreated DNA is amplified using a polymerase and at least one primer. In the next step, the amplified genomic DNA is hybridized to at least one oligonucleotide to form a duplex and extended by at least one nucleotide, wherein the nucleotide carries a detectable label and the extension of the methylation status of the respective cytosine in the genomic DNA sample depends. In the next step, the extended oligonucleotides are examined for the presence of the label. |