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publicationNumber EP-1909108-A2
titleOfInvention Systems and methods to perform assays for detecting or quantifiying analytes
abstract A method for processing the contents of multiple reaction receptacles to quantitatively determine the amount of a target nucleic acid present in each of said reaction receptacles, said method comprising the automated steps of a) successively providing to a heater a plurality of reaction receptacles, each reaction receptacle being provided with reagents sufficient to amplify and detect the presence of a target sequence, or its complement, contained within a target nucleic acid under the temperature conditions of said heater; b) periodically measuring the amount of a signal associated with the presence of an amplification product containing said target sequence or its complement in each reaction receptacle provided to said heater in accordance with step a); and c) quantifying the amount of said target nucleic acid present in each reaction receptacle based upon said measurements obtained in step b), wherein a portion of said plurality of reaction receptacles are being successively provided to said heater in accordance with step a) as the contents of a portion of said plurality of reaction receptacles are being processed in accordance with steps b)-c).
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