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publicationDate 2008-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1904522-A1
titleOfInvention Method for the purification of g-csf
abstract The invention relates to a method for obtaining the recombinant granulocyte-colony-stimulating factor (G-CSF), said method involving at least one cation-exchange chromatography step and at least one hydrophobic interaction chromatography step, the two chromatography steps directly succeeding each other in any order. The invention especially relates to a method for purifiyng G-CSF using a mixture of G-CSF and other proteins, said method comprising two cation-exchange chromatography steps which are respectively carried out before and after a hydrophobic interaction chromatography step.
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