http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1852702-A1

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filingDate 2006-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b82ad31e662bac035a4edf20d9193cfe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_191574523702fa9f3cd614495e01e01d
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publicationDate 2007-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1852702-A1
titleOfInvention Method for quantifying a cell population of interest contained in a human blood sample
abstract The present invention provides to a method for quantifying a cell population of interest contained in a human blood sample, comprising the steps of: contacting a sample of human blood with a cocktail comprising a first component, comprising at least one non-human antibody, antibody fragment or derivative thereof, capable of specifically binding to an antigen expressed on the surface of a first cell population distinct from a second cell population, said second cell population being the cell population of interest and being devoid of said antigen, complexed to a second component, comprising at least one non-human antibody, antibody fragment or derivative thereof, capable of specifically binding to the surface of human erythrocytes; separating antibody-labelled from unlabelled cells contained in the blood sample; collecting the supernatant comprising the second cell population; concentrating the second cell population contained in the supernatant and resuspending said cell population in a liquid capable of selectively lysing erythrocytes; concentrating the obtained cells, essentially removing all supernatant and resuspending said cells in a defined volume of physiological buffer; and determining the number of the cells of the second cell population contained in the sample. Furthermore, the invention provides to a kit for performing the method of the invention.
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priorityDate 2006-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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