http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1844141-B1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_26c536340aa6d174929c588553cce1db |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2006-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2012-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2e373a742161a8379a0a976a828c238 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c63d6380fec9fe313311ba22991f0f71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d49c8a691e013eaf2c27d2276b4a261 |
publicationDate | 2012-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1844141-B1 |
titleOfInvention | Method for separating and purifying nucleic acid |
abstract | A method for separating and purifying a nucleic acid comprising steps of: (1) adding a lysis solution to a biomaterial to prepare a sample solution containing a nucleic acid, and adding a water-soluble organic solvent or a solution containing a water-soluble organic solvent to the sample solution thereby preparing a sample solution containing the water-soluble organic solvent; (2) contacting the sample solution containing the water-soluble organic solvent with a solid phase thereby adsorbing the nucleic acid on the solid phase; (3) contacting a washing solution with the solid phase thereby washing the solid phase in a state where the nucleic acid is adsorbed on the solid phase; and (4) contacting a recovering solution with the solid phase thereby desorbing the nucleic acid from the solid phase, wherein, in the step (1), the water-soluble organic solvent or the solution containing the water-soluble organic solvent is added separately in at least two batches. |
priorityDate | 2005-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 132.